Tg(ASL1/Cryaa-Ralbp1)24Yca

This transgene consists of the delta en alphaA promoter, which consists of a heterogenic enhancer/promoter comprising the third intron of the chick delta1-crystallin gene and the mouse alpha crystalline promoter, a rabbit beta-globin intron, mouse Ralbp1 cDNA (2884 bp) and bovine growth hormone polyadenylation signal. This enhancer/promoter becomes active at the lens pit stage and remains active in all lens cells including both lens epithelial and fiber cells. Western blot and immunofluorescence confirmed expression in epithelial and fiber cells. Five lines were established: 3, 4, 24, 41, and 47. Line 24 and 47 have the highest numbers of transgene copies. (J:210388)