The targeting vector was designed to insert an internal ribosome entry site (IRES) fused to an enhanced green fluorescent protein (EGFP) sequence, and a loxP-flanked neomycin (neo) resistance cassette downstream of the stop codon. Cre-mediated recombination removed the floxed neo cassette.The endogenous coding sequence is not disrupted. (J:101977, J:210804)