设计了一种靶向载体,它包含在exon 1上游的单个loxP位点、一段frt标记的诺卡因抗性基因 cassette,然后是exon 1下游的另一个loxP位点。通过Cre介导的重组,去除了含floxed neo cassette和exon 1。 Western blot结果显示睾丸中没有检测到蛋白质,RT-qPCR分析证实了睾丸、肝脏和肺组织中没有转录本存在。(来源:J:210656)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/SvImJ
Targeted
插入,基因内删除
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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