Kit^{tm2.1(cre/Esr1*)Jmol}

A cDNA encoding MerCreMer (cre recombinase fused to two modified estrogen receptor ligand binding domains at both ends), and an frt-flanked neomycin selection cassette were cloned in-frame with the Kit ATG start site. Homologous recombination was performed in ES cells, and chimeras were bred to flp-expressing knock-in mice to delete the selection marker. (J:210584)