Tg(Igh-R4AVh/Ighm^a)1Dmnd

This transgene contains the unmutated, rearranged immunoglobulin V-D-J and heavy chain enhancer region from the anti-dsDNA producing hybridoma R4A, comprising the S107 V11 heavy chain gene and a Vk1 light chain gene, ligated to a 9.9 kb fragment from all (membrane and secreted) the exons of the Igh-mu constant region (Cu) from BALB/c, which is of the Ighma allotype. Southern blot analysis indicates that the transgene integrated in a single site of the genome. There are 5 copies of the transgene. (J:73871)