Mir205^tm1.2Mtm

A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. Flp-mediated recombination removed the reporter and selection cassettes. Cre-mediated recombination removed the microRNA sequence. (J:209028)