通过同源重组在ES细胞中,使用了靶向构建来替换Npr2基因的第1外显子,替换为Tamoxifen诱导的Cre重组酶(cre/ERT2)序列,后面跟着polyadenylation信号和Frt标记的neo选择标记。neo标记随后通过与表达Flp的 mice杂交来删除。(来源:J:205571)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S7/SvEvBrd-Hprt1b-m2
Targeted
插入,基因内删除
--
1
3
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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