在exon 8的上游插入了一个loxP位点。下游的exon 9位置插入了一个FRT标记的诺卡因抗性基因座,带有内含子启动子(IRES)和一个5'的loxP位点作为splice捐赠者,也带有loxP位点。通过Flp介导的重组,选择性基因座被去除,exons 8和9被标记为可剪接。 Western blot分析证实了小鼠胚胎提取物中没有蛋白质表达(来源:J:204996)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入,基因内删除
--
1
2
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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