靶向载体设计为在exon 2上游插入一个loxP位点,接着是含frt标签的诺卡因抗性(neo)基因座,然后在exon 2下游再增加一个loxP位点。通过与Tg(CMV-cre)1Cgn小鼠杂交,产生了突变小鼠,以去除包括exon 2在内的含loxP的区域。(来源:J:207523)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count