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中文
一种"TetOP-Cre翻转插入质粒"（包含一个splice接受器-polyA序列和四环素响应元件(tet))，与CMV基本启动子相连，位于Cre重组酶 cassette上游，被用来处理在Col1a1基因3' UTR中插入了FRT-PGK-neo-hygro-FRT cassette的ES细胞。这个"翻转插入"质粒与编码Flpe的质粒一起注射，通过Flpe介导的重组，将tetO-cre序列插入Col1a1基因的3' UTR。Col1a1tm13(neo/hygro)Jae ES细胞还包含Gt(ROSA)26Sortm1(rtTA*M2)Jae这一突变基因。通过使用多目标的ES细胞产生嵌合体和生殖动物，通过给予多西环素(doxycycline)处理，rtTA*M2能结合TetOP启动子，进而驱动cre的表达。(来源：J:207446)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
