在胚胎中,通过Cre介导的重组,2号和3号外显子被一个floxed诺卡因抗性 cassette替换。 Western blot结果显示表达了一种截短的蛋白。(来源:J:207249)
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Exons 2 and 3 were replaced with a floxed neomycin resistance cassette. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the expression of a truncated protein in embryos. (J:207249)