将Cre重组酶的C端片段(60-343aa)与DnaE分裂 intein序列的intein-C肽的36端氨基酸C末端部分融合,然后插入包含人enhancer 218序列和Hsp68微弱启动子及lacZ报告基因的载体中。构建最终包含了将lacZ基因和Ccre-intein-C区分开的间隔序列。通过将线性化的构建物注入囊胚的核中,产生了转基因供体。文中未提及具体线号。(来源:J:207270)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Not Specified
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
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Year
IF
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