Pld1^tm1.1Gdp

A loxP site was inserted upstream of exon 13. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 14. This allele is created by using either Cre-mediated recombination to remove exon 13, exon 14 and the selection cassette or Flp-mediated recombination to remove the FRT-flanked neo cassette, followed by Cre-mediated recombination to remove exons 13 and 14 . Western blot analysis confirmed the absence of protein expression in the liver and mouse embryonic fibroblasts. (J:207283)