KH2 ES细胞通过插入了"Gt(ROSA)26Sortm1(rtTA*M2)Jae"和"Col1a1tm13(neo/hygro*)Jae"基因,然后使用了带有tetacycline响应元件(TRE或tetO)以及FLAG标记Fos cDNA的"flp-in plasmid"进行重定向。为了实现3' UTR区域的整合,还有一份flpe重组质粒用于辅助。这项工作参考了文献(J:201145)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
--
1
24
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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