Fus^em2Rkuhn

TALEN targeting of exon 15 for homologous recombination with a synthetic oligodeoxynucleotide induced a point mutation (C to T) that results in the amino acid substitution of leucine for phenylalanine at position 517 (P517L). Founder F36 exhibit the intended point mutation and an additional nucleotide substitution G to A in the TALEN-Fus15B targeting sequence. (J:201579)