设计了一个通过重组酶介导交换(RMCE)载体,其序列从5'至3'如下:无启动子的诺瓦克抗药性基因座,接着是3个SV40poly(A)序列(3xpA),然后反向插入一个包含3xIns(鸡β球蛋白绝缘子的400bp核心区域)的片段,以及CAGGS(CMV-IE增强子/鸡β-actin/兔β球蛋白)的融合启动子,带有floxed-3xpA标签,最后是连接了Rpl10a(ribosomal蛋白L10a)编码的增强绿色荧光蛋白(EGFP)的cDNA。载体中的loxP标记的pA座旨在阻断EGFP/Rpl10a的转录。来源:(J:206130)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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