在J:204701中,一个带有核定位序列和新霉素抗性基因座的lacZ基因替换掉了第二个外显子。 Western blot分析证实了肺和肾组织中没有蛋白质表达。
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A lacZ with a nuclear localization sequence and neomycin resistance cassette replaced the second exon. Western blot analysis confirmed the absence of protein expression in the lung and kidney. (J:204701)