在exon 3的上游插入了loxP位点,而在exon 3的下游则插入了FRT和loxP位点。通过Cre介导的重组,去除了exon 3。 Western blot分析证实了E9.5胚胎中没有蛋白质表达。(来源:J:203698)
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A loxP site was inserted upstream of exon 3. An FRT and loxP site were inserted downstream of exon 3. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of protein expression in E9.5 embryos. (J:203698)