将编码cre/ERT2的同源二聚体番茄cDNA(tdTomato)和人类生长激素内含子及polyadenylation位点插入Axin2基因座,位于起始密码子下游,通过ES细胞的同源重组。然后通过与表达Flp1的转基因小鼠杂交,删除neo选择标记 cassette。(来源:J:204142)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count