Vhl^tm1.1Whkr

Exon 1 was replaced with a modified exon 1 in which nucleotide substitutions (ATG to CTA) results in the amino acid substitution of leucine for methionine at position 1 (p.Met1Leu). This mutation prevents the expression of the long isoform. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted downstream of the modified exon 1. Western blot analysis confirmed the absence of long isoform expression and increased expression of the short isoform in the kidney and liver. (J:198361)