在基因中插入了新霉素抗性基因元件, Western blot 检测证实了呼吸道、鼻软骨和膝关节中没有蛋白表达(来源:J:203141)
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A neomycin resistance cassette was inserted into the gene. Western blot analysis confirmed the absence of protein expression in the trachea, nasal cartilage and knee cartilage. (J:203141)