带有polyadenylation序列和floxed neomycin抗性 cassette的EGFP,它替换掉了2到7号染色体的外显子, Western blot结果显示精子中没有检测到蛋白表达。(来源:J:203477)
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An EGFP with a polyadenylation sequence and a floxed neomycin resistance cassette replaced exons 2 through 7. Western blot analysis confirmed the absence of protein expression in the sperm. (J:203477)