Mapkapk2^tm1.1Yaff

A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site (in reverse complement sequence to the first loxP site) downstream of exon 2 as well as a frt:PGK-neo:frt sequence downstream of the second loxP site. Because inward-facing loxP sequences result in cre-mediated inversion rather than excision, exon 2 can reversibly switch from Mapkap2 expression orientation (MK2<+CV>, CV; Cre-versible) to the null orientation (MK2<-CV>) and back as long as Cre recombinase is active in the cell.FLP recombinase mediated recombination removed the neomycin cassette. (J:203686)