一个敲除的诺卡因抗性基因座替换了exon 1。通过Cre介导的重组,去除了诺卡因抗性基因座。 Western blot分析证实了睾丸中没有蛋白质表达。(来源:J:201474)
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A floxed neomycin resistance cassette replaced exon 1. Cre-mediated recombination removed the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in the testis. (J:201474)