在编码序列上游插入了带有STOP cassette的元件,这阻断了蛋白表达。 Western blot结果显示没有检测到蛋白质的存在。(来源:199647)
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A floxed STOP cassette was inserted upstream of the coding sequence that blocks expression. Western blot analysis confirmed the absence of protein expression. (J:199647)