插入载体包含一个带有lacZ截断序列、polyadenylation信号以及GFP标记的人源H2B基因,最后是FRT引导的诺卡因抗性筛选元件,它被插入在exon 1的起始密码子上游6个碱基的位置。在正确靶向的细胞中表达的Flp重组酶会去除这个筛选元件。只有在lacZ截断序列被去除后,标记的H2B基因才会表达。(来源:J:198628)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入,基因内删除
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1
5
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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