Red/ET recombination was used to insert the sequences encoding cre recombinase, the SV40 poly(A) signal, and a kanamycin resistance gene at the ATG (translational initiation site) of the Slc32a1 (Vgat or Viaat) locus contained in RP23-392-P11 BAC clone derived from C57BL/6 mouse genomic DNA. The purified construct was injected into pronuclei of C57BL/6 oocytes. Transgenic line 65 was used in further analyses. (J:202863)