在exon 2上游插入了loxP位点。在exon 4下游插入了一个3'有loxP标记的诺卡因抗性 cassette,它带有FRT序列。通过Flp介导的重组移除了诺卡因抗性 cassette,使得exons 2到4成为可删除的。随后,Cre介导的重组去除了exons 2到4。 Western blot结果显示睾丸中没有检测到蛋白质表达。(来源:J:197673)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S6/SvEvTac x C57BL/6J)F1
Targeted
插入,基因内删除
--
1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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