Igs2^{tm2(ACTB-tdTomato,-EGFP)Zng}

The "multiple lox" MADM-ML-11 targeting vector has a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "TG MADM-ML" cassette. The "TG MADM-ML" cassette is designed with the N-terminal portion of a red fluorescent protein (tdTomato; nucleotide 1-3), a beta-globin intronic sequence (containing an frt-lox5171-lox2272-frt-loxP-Neo-loxP), the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; nucleotide 274-724), and an SV40 T-antigen poly(A) signal. (J:101977, J:202665)