Recombinase mediated cassette exchange replaced the hygromycin resistance casssette in Gt(ROSA)26Sortm1Rkuhn with a neomycin resistance cassette, polyadenylation sequence, FRT site and a C-terminally V5-tagged human cDNA lacking the mitochondrial leader sequence (-MLS; FHcys) with a polyadenylation sequence driven by a CAG promoter. (J:198486)