The construct replaced the sequence around the transcription initiation site with a loxP site, a Pax7 cDNA (Pax7d) with a polyadenylation sequence, FRT site, neomycin resistance cassette with polyadenylation sequence, loxP site, a GFP gene and mouse chromosome localizing histone H2B fusion (H2BGFP) with a polyadenylation sequence, and an FRT site. The Pax7 cDNA failed to express. (J:200727)