Akap9^{tm1b(KOMP)Wtsi}

The L1L2_gt0 cassette was inserted at position 4018123 of Chromosome 5 upstream of the critical exon 8 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 8 at position 4019078. The critical exon 8 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Cre-mediated recombination removed exon 8 and left the reporter construct intact. Western blot analysis confirmed the absence of protein expression in the pancreas and testis. (J:198499)