A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), and a nuclear localized mCherry in in-frame after the last codon of the Gad2 gene. A frt-flanked neo was places downstream of the mCherry. Flp-mediated recombination removed the neomycin resistance cassette. (J:221246)