Braf^tm2.2Urr

A loxP site was inserted upstream of exon 3. A floxed neomycin resistance cassette was inserted downstream of exon 3. Cre-mediated recombination removed exon 3 and the neomycin resistance cassette. Western blot analysis confirmed the absence of the two predicted protein isoform expression in embryos. This allele is predicted to produce a truncated, non-functional protein. (J:199842)