在exon 1上游插入了loxP位点。在exon 1下游插入了loxP和FRT标记的诺卡因抗性元件。通过Flp介导的重组移除了诺卡因抗性元件,留下exon 1是可删除的。随后,通过Cre介导的重组去除了exon 1。 Western blot结果显示,小鼠胚胎成纤维细胞中没有蛋白质表达。(来源:199753)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129
Targeted
插入,基因内删除
--
1
--
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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