Tg(CAG-XBP1*/cre/ERT2)#Grtn

The pCAX-F-XBP1/deltaDBD-venus plasmid construct was modified by replacing the venus cDNA with cDNA encoding a cre/ERT2 fusion protein. The final construct consists of nucleotides 410-633 of a human XBP1 cDNA including a Kozak sequence and FLAG-tag coding sequence fused to cre/ERT2 cDNA under control of a CAG promoter construct. The XBP1 fragment lacks the DNA binding domain but includes a 26-nt endoplasmic reticulum stress-specific intron. Under normal conditions, the mRNA of the fusion gene is not spliced and translation terminates at a stop codon near the junction of XBP1 and cre/ERT2. During ER stress, the 26 nt intron is spliced out and the XBP1/cre/ERT2 mRNA is transcribed. Founder and line number information is not available. (J:197481)