构建了一个靶向载体,包含cre的cDNA和由弗特(Frt)序列介导的氨苄西林抗性筛选 cassette,周围是Trp63同源臂。经过电穿孔,这个载体被导入ES细胞中进行同源重组。这导致cre序列的起始密码子ATG插入到Trp63基因座,取代了原有的起始密码子(来源:J:197339)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S/SvEv
Targeted
插入
--
1
16
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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