An RAKR furin motif sequence to trim the residual 2A peptide from the upstream protein, glycine-serine-glycine (GCG) spacer, foot-and-mouth disease virus 2A peptide (F2A) sequence, EGFP and FRT-flanked neomycin resistance (neo) cassette were inserted into intron 2. Flp-mediated recombination removed the neo cassette. (J:198603)