The targeting contruct inserted (5' to 3') a wild-type FRT site, splice acceptor site, floxed neomycin resistance/STOP cassette, reverse tetracycline transactivator (rtTA), IRES, puromycin resistance cassette, polyadenylation sequence, tetracycline response element (TRE), a polycistronic Pou5f1, Sox2, Klf4, and Myc transgene, IRES, EGFP, luciferase, polyadenylation sequence, mutated FRT site and promoterless neomycin resistance cassette. (J:195147)