Exon 1被标记了。下游插入了一个FRT引导的neo cassette。 Western blot结果显示肝脏、脾脏和睾丸中蛋白质表达量降低。(来源:198845)
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Exon 1 was floxed. An FRT-flanked neo cassette was inserted downstream of exon 1. Western blot analysis confirmed reduced protein expression in the liver, spleen and testis. (J:198845)