在exon 2的开放阅读框位置,插入了一个敲除neo抗性基因的载体。通过Cre介导的重组,去除了neo抗性基因 cassette。 Western blot结果显示大脑中没有检测到蛋白质表达。(来源:J:196042)
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A floxed neomycin resistance cassette replaced the open reading frame in exon 2. Cre-mediated recombination removed the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in the brain. (J:196042)