Gt(ROSA)26Sor^{tm10.1(Tcf/Lef-CFP)Mgn}

The vector was made on a backbone of a basal construct containing 5.166 kb from Rosa26 region that was absent in the Rosa26LCA allele, tandem Lox71/Lox2272 sites and a flrted (flanked by FRT)Pgk-Neo cassette for positive selection of ES cells after RMCE. Within the Rosa26 gene sequences the region from -228 to +81 were replaced by a Wnt response element (7xTcf/Lef binding site) fused to a TATA-Cerulean (CFP) reporter. The chimeric mice were crossed with Flpe mice to remove the neomycin resistance gene. (J:195112)