在exon 5的1.3kb编码序列中,插入了一个新霉素抗性基因 cassette,这占了编码序列的70%。 Western blot结果显示大脑中没有检测到蛋白表达(来源:J:194336)
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A neomycin resistance cassette replaced 1.3 kb of the coding sequence in exon 5, which comprises 70% of the coding sequence. Western blot analysis confirmed the absence of protein expression in the brain. (J:194336)