Igs6^{tm2.1(ACTB-EFGP,-tdTomato)Luo}

A targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "GT MADM" cassette. The "GT MADM" cassette used here was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire "GT MADM" construct was inserted into the John12 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). Cre-mediated recombination removed the floxed neo cassette. (J:194079)