在融合的exon 6-7下游,插入了一个floxed neo cassette。通过Cre介导的重组,去除了neo cassette。 Western blot结果显示,仅检测到了长型isoform的表达。(来源:J:196531)
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A floxed neo cassette was placed in the intron downstream of the fused exon 6-7. Cre-mediated recombination removed the neo cassette. Western blot analysis confirmed only expression of the long isoform. (J:196531)