在第七外显子上游插入了LoxP位点,随后在第七外显子下游插入了带有floxed neo cassette的片段。通过Cre介导的重组,删除了第七外显子和neo cassette。 Western blot结果显示,仅检测到了短型isoform的表达。(来源:J:196531)
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A loxP site was inserted upstream of exon 7. A floxed neo cassette was inserted downstream of exon 7. Cre-mediated recombination removed exon 7 and the neo cassette. Western blot analysis confirmed only expression of the short isoform. (J:196531)