将含有GFP/cre/ERT2(绿色荧光蛋白、Cre重组酶和雌激素受体1tamoxifen结合域融合基因,以及FRT标记的PGK-NEO筛选 cassette)的靶向载体插入Prg4(蛋白聚糖4,巨核细胞刺激因子,关节表皮区蛋白)外显子2的起始密码子位置。生成的嵌合动物与表达FLP重组酶的ACT-FLPe小鼠(未知遗传背景)杂交,以去除FRT标记的PGK-NEO筛选 cassette。(来源:J:197163)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count