Tg(Chrna2-cre)1Kldr

A construct (composed of cre recombinase with a nuclear localization signal, SV40 polyadenylation sequence, and an Frt-Kanamycin/neo-Frt cassette) was introduced into the ATG site in exon 2 of the Chrna2 gene in a bacterial artificial chromosome (BAC RP23-48P22) by recombineering in bacterial cells. Modified BAC DNA was purified and injected into mouse pronuclei to produce transgenic mice. One founder line showed cre expression in cells that express the CHRNA2 protein. (J:193558)