第四外显子被标记了Cre介导的重组,它去除了第四外显子,并在第四外显子上游插入了FRT引导的诺卡因抗性基因座。 Western blot结果显示神经视网膜中没有蛋白质表达。(来源:J:196540)
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Exon 4 was floxed. Cre-mediated recombination removed exon 4 and the FRT-flanked neomycin resistance cassette inserted upstream of exon 4. Western blot analysis confirmed the absence of protein expression in neural retina. (J:196540)