Tg(Scx-EGFP)1Shuk

A construct was generated with enhanced green fluorescent protein (EGFP) coding sequence under control of approximately 11 kb of the Scx regulatory region (~4kb of upstream sequence and ~5 kb of downstream sequence, retrieved from a bacterial artificial chromosome RP23-415D19). This construct was used for microinjection to produce transgenic mice. Two transgene positive founders were obtained with one line being established and used for analyses. GFP was expressed in the limb buds, the syndetome, and the branchial arches at E10.5. The expression pattern of GFP was comparable to endogenous Scx expression. (J:195053)